Romelu Lukaku and Darron Gibson joined up with Belgium and Republic of Ireland respectively but were released back to Everton due to injury.
Luka has a slight hamstring injury and Gibbo has a a stomach strain both picked up in the 2-1 win at QPR.
Both players should not be out for long and may have a chance to be ready for Southampton.
Roberto said: "Romelu had his scan with the Belgian set-up and he's going to be receiving treatment for the next few days, and we'll see how that evolves.
"It's a bit of a hamstring strain due to the amount of games that we've played and the amount of fatigue we've accumulated in those games.
"We're quite positive because of the way Rom usually reacts to these types of treatments and how fit he is as an individual.
"It's too early to say how long he's going to be out or if he's going to miss any games but he's concentrating on getting back fit to train with the group.
"With Darron, he had a little bit of a stomach strain. He made sure that he finished the game but he felt it in the last 10-15 minutes. After the game, when his body cooled, he then felt a real difficulty walking and trying to exercise on it.
"He's been receiving treatment and obviously he met with the Irish party, and now we're going to assess him and see how long it will be before he can join the group again.
"In both cases, I don't think they are major injuries but they are injuries that need to be well looked after and we'll see if both players are available or not for the Southampton game."
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